Timeline expectations should be modest. If niacin is correcting a true shortfall, improvements in appetite, coat, or general steadiness may be noticed over weeks, not days. Niacin administration can influence measurable levels in plasma and urine (Menon RM, 2007), but numbers are not the same thing as visible wellbeing.
If you don’t see a meaningful change, that information is still useful: it suggests the limiting factor may not be vitamin B3. At that point, a broader aging-support approach—sleep, mobility, hydration, stress reduction, and a well-designed supplement strategy—often fits better than escalating a single nutrient.